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. Author manuscript; available in PMC: 2018 Apr 21.
Published in final edited form as: Matrix Biol. 2018 Mar 3;67:32–46. doi: 10.1016/j.matbio.2018.01.012

Fig. 4.

Fig. 4

LN domain subunit specificity of assembly on cells. SCs were incubated with 28 nM Lmβ1ΔLN (A), Lmα1ΔLN (B) or Lmγ1ΔLN (C) alone or coupled to βLNNd, αLNNd or γLNNd. After 1 h the cells were washed, fixed and immunostained with Lmα1-specific antibody with a dapi counterstain. Immunofluorescence intensities/cell were determined and are shown in adjacent plots (average ± SD, n = 7–11 fields/condition). Bars marked with asterisks within each graph significantly differ (P < 0.0001) from unmarked bars. Laminin accumulation was dependent on pairing the βLNNd with Lm β1ΔLN, αLNNd with Lmα1ΔLN, and γLNNd with Lmγ1ΔLN, i.e. assembly of complexes required a full complement of α, β and γ LN domains.