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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Muscle Nerve. 2018 Jan 8;57(5):814–820. doi: 10.1002/mus.26025

Figure 3. Effects of NT-1654 on AChR in the muscle of EAMG rats.

Figure 3

Muscle tissue sections of the tibialis anterior were obtained at day 49 after immunization and stained with α-bungarotoxin (red) to label AChR and antibodies against NF-H/SV2A (green) to label nerve branches and terminals. (A) Representative images (10x magnification) show AChR and nerve endings of healthy control, EAMG + vehicle, EAMG + NT-1654 (2 mg/kg), and EAMG + NT-1654 (6 mg/kg) rats in tibialis anterior tissue. Scale bar: 100 μm. (B–D) Summarized results show that NT-1654 improved AChR covering the nerve terminals in EAMG rats. Normalized pixel density of AChR relative to healthy controls (B), normalized pixel density of AChR and nerve terminals relative to healthy controls (C), and the percentage of AChR covering nerve terminals relative to total AChR (D). Data are expressed as mean ± s.e.m.; n = 6 rats per group; *p<0.05; **p<0.01.