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No consistent effect of shRNA mediated knock-down of AHNAK, ALIX and TSG101 on the number of vector genome RNA copies (HV) in 293T-GIPZ cells compared to 293T-GIPZ-scramble. Comparative quantification of HV RNA fold difference in cell lysates using Q-RT-PCR is shown. Results of vector genome RNA copy levels in two sets of samples; normalised to β-actin copies per cell; negative control 293T-GIPZ-scramble and ‘no shRNA’ are comparable.
