Table 1.
Coverage of viral protein sequences by LC-MS/MS in purified vectors.
| Viral protein | Protein length (amino acids) | MS set 3 |
MS set 3 rejection |
MS set 3 |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RDpro-GFP (240x) | RDpro-Empty (240x) | RDpro-GFP (240x) | RDpro-Empty (240x) | VSV-G GFP (40x) | VSV-G Empty (40x) | Gag/Pol-GFP (40x) | VSV-G only (240x) | |||
| Gag | MA | 130 AA | 45% | 46% | 63% | 66% | 73% | 96% | 67% | ND |
| CA | 215 AA | 57% | 57% | 79% | 61% | 49% | 50% | 52% | ND | |
| p2 | 13 AA | ND | ND | 100% | 100% | ND | ND | ND | ND | |
| p6 | 56 AA | ND | ND | ND | ND | 27% | 18% | ND | ND | |
| Pol | PR | 98 AA | ND | ND | 21% | ND | 17% | ND | 18% | ND |
| RT | 559 AA | 3% | 5% | 33% | 21% | 17% | 17% | 17% | ND | |
| IN | 287 AA | ND | ND | 15% | 9% | 15% | 9% | 1% | ND | |
| VSV-G Env | 411 AA | ND | ND | ND | ND | 20% | 22% | ND | 41% | |
| RDpro Env | 565 AA | ND | ND | 4% | 7% | ND | ND | ND | ND | |