Fig. 3.

Post-transcriptional regulations of potential miR-217-5p target genes. To experimentally validate miR-217-5p mediated regulation of potential target genes, HCT 116 cells were transfected with miR-217-5p mimic or non-targeting siRNA (NT) as described in Fig. 2. After 48 h, potential target gene expression was analyzed by qRT-PCR (a). The relative mRNA expression of potential target genes was normalized to PPIA and NT. In light blue highlighted candidate genes of (a) were further analyzed by Western blot (b – representative blots; n = 3). Densitometric analyses of the Western Blot were performed using the software FusionCapt Advance. GAPDH was used as loading control. The expression of the respective protein was normalized to NT. Differences in the expression of mRNA or target proteins between miRNA mimic transfected cells and NT transfected cells were accessed using unpaired t-test [n = 3 biological and technical replicates; mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001]