Fig. 5.

PRKC1, ITGAV, BAG3 and MAPK1 as direct targets for miR-217-5p in HEK293T cells. Relative luciferase activity 3 days after co-transfection of the pMirGLO vector with the binding sites 1 to 4 and the fusion of binding sites 2 and 3 of PRKC1 (a), the binding sites of ITGAV (b) and BAG3 (c) as well with the binding sites MAPK1_1 to 6 and the fusion of binding sites 1–4 (d) with miR-217-5p mimic, miRNA inhibitor anti-miR-217-5p or non-targeting siRNA (NT). Statistical differences between means were tested using unpaired t-test [n = 3 biological and technical replicates; mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001]