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. 2018 Apr 20;8:6307. doi: 10.1038/s41598-018-24892-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and Alkaline Phosphatase activity measurements, in vitro. (A) ß-TCP scaffold granules. (B) AT-MSCs were seeded on ß-TCP granules and stained with DAPI. Red arrows show cell nuclei and black arrows show the pores in the scaffold. (C) Effect of electrical stimulation on the viability of AT-MSCs seeded on ß-TCP scaffold, during 21 days in culture. Cell viability measured by MTT assay, shown as fold change relative to day 0. There was no significant difference in viability between cells exposed, and not (controls) exposed to electrical stimulation. (D) Effect of electrical stimulation on Alkaline Phosphatase activity of AT-MSCs during 21 days. Data are presented as mean ± SD (n = 3), (*p < 0.05).