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. 2018 Apr 20;8:6307. doi: 10.1038/s41598-018-24892-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bone healing score and tissue constituent percentage of the defect. At eight weeks post-surgery, (A) Healing scores were calculated using histological image analysis. Data are shown as mean ± SD (n = 5). Experimental group scored significantly higher than control and sham groups, (#p < 0.1; *p < 0.05; **p < 0.01) (B) Histomorphometric distribution of newly formed bone, fibrous, and cartilage tissues within the defect, at eight weeks post-surgery. Percentage measured from the whole defect area using ImageJ. Data are shown as mean ± SD (n = 5).