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. 2018 Apr 20;8:6307. doi: 10.1038/s41598-018-24892-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Osteogenic marker gene expression, in vivo. At eight weeks post-surgery, osteogenic marker gene expression measured by means of qRT-PCR and normalized to RPLP1 and YWHAZ (housekeeping genes). (A) TGF-ß1, (B) BMP2, (C) RunX2, (D) ColIa2, (E) Osterix, (F) Osteopontin and (G) Calmodulin expression were significantly higher at eight weeks in the experimental in comparison to sham and control groups. Data are presented as mean ± SD (n = 5), (*p < 0.05).