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. 2018 Apr 20;8:6331. doi: 10.1038/s41598-018-24576-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fibronectin and KLK12 are both expressed in the vascular endothelium. Immunofluorescent staining of capillaries formed after 9 days of fibroblast-EC co-culture. Top panel: Staining of KLK12 (green), PECAM/CD31 (red), and nuclei (blue). Bottom panel: Staining of FN (green), PECAM/CD31 (red), and nuclei (blue). Colocalization of KLK12 or FN with capillaries (CD31/PECAM staining) appears in yellow. Scale bars correspond to 50 µm for 20x images and 30 µm for 40x images.