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Vacuolar targeting and slow secretion are independent of the assembly state of SIgA/G. Protoplasts from leaves of transgenic tobacco plants expressing IgA/G, dIgA/G, or SIgA/G were pulse-labeled for 1 h and chased for the indicated periods of time. Cells and the corresponding incubation media were homogenated, subjected to immunoprecipitation with anti-IgG antiserum, and analyzed by 6% (w/v) non-reducing SDS-PAGE and fluorography. Numbers at left indicate molecular mass markers in kD.
