Figure 4.
Characterization of P1156. (A) Colloidal Coomassie stained SDS-polyacrylamide gel showing size exclusion column fractions (lanes 3–6) obtained following nickel affinity chromatography for P1156. Molecular weight ladder (kDa) is shown in lane 1. The gel image was cropped for clarity and the uncropped image is provided in Fig.S5. (B) Biolayer interferometry sensorgrams of P1156 binding to indicated chemokines. Plots display wavelength shift (y-axis, nm) versus time (x-axis, seconds). Solid lines indicate collected data, dashed lines indicate fitted data.
