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. 2018 Apr 20;8:6327. doi: 10.1038/s41598-018-24154-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative titration calorimetric curves from ITC experiments conducted at 37 °C. Top graphs show the injection peaks, with each peak corresponding to the injection of 1.96 µL (except that 0.48 µL was used for the first peak) of 20 mM POPC:POPG (80:20 % (mol/mol)) lipid vesicles into 177 µL peptide in aqueous medium, pH 7.4 (70 µM for fluorophore-labeled PEN and 40 µM for PEN). Bottom graphs show the corresponding integrated injection heats as a function of lipid-to-peptide (L/P) molar ratio. The solid lines are the best fits to the data.