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. 2018 Apr 20;8:6337. doi: 10.1038/s41598-018-24706-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of MIF absence on colon epithelial cells. The expression of E-cadherin (a), relative to tubulin expression was measured by western blot in epithelial cells of the large intestine. Representative blot is shown on the right-hand side along with immunostaining for E-cadherin of colon sections. The mRNA expression of tight junction proteins (b) was determined by real-time PCR and normalized to β-actin expression. IL-18 (c) and IL-1β (d) protein expression was measured by western blot as in (a). Representative blot images are shown below the graphs. Presented results are average ± SD from 16 animals per group, obtained in 3 separate experiments. For statistical analysis, test of normality and Mann–Whitney U-test were used. *p<0.05 represents the significant difference between values of protein and mRNA expression in epithelial cells of WT vs MIF-KO mice.