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. 2018 Apr 20;8:6335. doi: 10.1038/s41598-018-24726-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isoflavone extract inhibited TNF-α- and IL-17A-induced NF-κB phosphorylation in human primary keratinocytes. Primary keratinocytes were pretreated with different doses of isoflavone extract for 24 hours and then treated with TNF-α or IL-17A. After treatment with TNFα (A) or IL-17A (C), with the quantification data shown in the right panel (B,D), the total protein was extracted from the cells and IκBα expression was determined via western blotting. Data were the mean ± SEM from at least three independent experiments. *P < 0.05, indicating significant effects of isoflavone extract as compared to control.