Fig. 6.

Nicotinic acetylcholine (nACh) receptor inhibitors cancel the effect of nicotine on LPS-increased proton current in microglia. a Current traces from −100 to +100 mV from the holding potential of −60 mV for 1 s are shown. Application of LPS (1 μg/ml) was for 24 h and nicotine (1 μM) was pre-treated for 1 h before application of LPS. Methyllycaconitine (MLA, 100 nM) and α-bungarotoxin (α-Bgt, 100 nM) were pre-treated for 30 min before application of nicotine, followed by LPS application. b I–V relationships in control (filled square), with 1 μg/ml LPS (filled circle), LPS with Nic (filled upright triangle), pre-treated with MLA (filled downright triangle) and α-Bgt (open diamond) are shown. c The relative current amplitudes at +100 mV in b are shown. **p < 0.01, ***p < 0.005 compared to LPS + Nic