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. 2018 Apr 20;15:118. doi: 10.1186/s12974-018-1155-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — BLVRA nuclear translocation was dependent on eNOS-derived NO. a NO production was measured in splenic tissues treated with BLVRA and co-administered with eNOS inhibitor and iNOS inhibitor, respectively. Western blot analysis of BLVRA expression in the nucleus of spleen tissues treated with BLVRA + eNOS inhibitor (b), BLVRA + NO donor (SNP) (c). d Western blot analysis of BLVRA and TLR4 expression in the cytoplasm treated with BLVRA and BLVRA + eNOS inhibitor. e–g Representative quantitative analysis were shown respectively. Values are expressed as mean ± SD. ^*P < 0.05 compared with sham, ^#P < 0.05 compared with GMH + vehicle. ^&P < 0.05 compared with GMH + BLVRA. N = 6 each group