Figure 4.

(A) Influence of Ca²⁺ concentration on SAL4 activity. The star symbol indicates the absence of lipase activity checked in the absence of CaCl₂ and in the presence of 10 mM EDTA. (B) Influence of metal ions concentrations (2 mM each) on SAL4 activity was studied using olive oil emulsion as substrate under optimal conditions of pH and temperature. The control represents 100% of lipase activity in the absence of metal ions under the same condition. Values represent the mean of three replicates.