Table 1.
Flow sheet of the SAL4 purification.
| Purification step | Totala activity (units) | Proteinb (mg) | Specific activity (U/mg) | Activity recovery (%) | Purification factor |
|---|---|---|---|---|---|
| Extraction | 32,000 | 362 | 88.4 | 100 | 1 |
| (NH4)2SO4 Precipitation (25–65%) | 25,600 | 115 | 222.6 | 80 | 2.5 |
| Heat treatment (70 °C) | 22,040 | 18.5 | 1191.3 | 68.9 | 13.5 |
| RP-HPLC (C-18) | 16,594.5 | 2.3 | 7215 | 51.7 | 81.4 |
a
1 Unit: μmole of fatty acid released per min using olive oil emulsion as a substrate in the presence of 1 mM NaDC and 2 mM CaCl2.
b
Proteins were estimated using the Bradford method (Bradford, 1976). The experiments were conducted three times.