Table 3.
Lipase stability of SAL4 and the commercial detergent Lipolase® in the presence of surfactants, detergents and oxidizing agent.
| Detergent components | Residual activity (%) |
|
|---|---|---|
| SAL4 | Lipolase® | |
| None | 100 | 100 |
| Surfactants (1%) | ||
| Tween-20 | 102.3 ± 2.5 | 82.4 ± 1.6 |
| Tween-80 | 100.7 ± 2 | 72.71 ± 2.3 |
| Triton-X100 | 100.7 ± 1.2 | 49.67 ± 4.2 |
| SDS | 86.7 ± 1.7 | 69 ± 1.2 |
| NaDC | 110 ± 2.5 | 87.9 ± 3.1 |
| NaTDC | 108 ± 1.7 | 80.2 ± 2 |
| Oxidizing agents (1%) | ||
| Hydrogen peroxide | 89.3 ± 4 | 55.3 ± 2.5 |
| Sodium perborate | 91 ± 3.6 | 61.3 ± 4.2 |
| Sodium hypochlorite | 79 ± 2.5 | 52 ± 2 |
| Commercial detergents | ||
| Solid detergent (5mg/mL) | ||
| Ariel | 100 ± 0 | 63 ± 3.6 |
| Dixan | 97.7 ± 2.5 | 59.7 ± 5.5 |
| Nadhif | 92.3 ± 2.1 | 49.3 ± 4 |
| Tide | 87 ± 3 | 53 ± 4.3 |
| Liquid detergent (1/100) | ||
| Dixan | 85. 7 ± 4 | 54 ± 1.5 |
| Nadhif | 85. 7 ± 1.2 | 46 ± 3.5 |
| Dac | 88.3 ± 2.5 | 52 ± 1.7 |
| Fairy | 83.3 ± 3.5 | 80.3 ± 1.5 |
SAL4 preparation was incubated with different detergents components for 60 min at 40 °C and the remaining activity was measured under standard assay conditions. Residual SAL4 activity was determined at pH 12 and 60 °C. Results are the relative lipase activity expressed as the percentage of the maximum activity recorded without the addition of compound; data are means of triplicate determinations ± standard deviation.