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miR-25 Tough Decoy Generation and Evaluation
(A) miR-25 TuD was generated. (B) Single injection of miR-25 antagomir over 8 weeks showed significantly reduced relative mature miR-25 expression compared to AAV9 miR-25 TuD. (C) Co-transfection of miR-25 TuD with pre-miR-25 significantly abolished the restoration of SERCA2a, and transfection of miR-25 TuD in H9c2 showed significantly attenuated miR-25 expression. All data are represented as means ± SD (**p < 0.01, ***p < 0.005).
