Figure 4.

SERCA2a Expression Is Restored by miR-25 TuD

(A) miR-25 TuD transfer restored SERCA2a expression. Mature miR-25 expression is significantly induced in HF with AAV9 control-transferred mice and significantly reduced in HF with miR-25 TuD-transferred mice. (B) Northern blot of miR-25 was normalized by U6. Polyacrylamide gel before transfer shows 8S, 18S, and 28S bands clearly, showing limited degradation. Quantification shows that miR-25 increases as HF progresses. However, after AAV9 miR-25 TuD transfer, miR-25 levels significantly decrease. (C) qRT-PCR data show that miR-25 expression increases in HF mice with AAV9 control transfer and decreases in HF mice with AAV9 miR-25 TuD transfer. Threshold cycles are plotted against the number of RNA concentration as a standard curve. All data are represented as means ± SD (*p < 0.05, **p < 0.01, ***p < 0.005).