Figure 6.

Administration of PEG-CBS Rescues the Structure of the Ciliary Zonule in the Eyes of I278T Mice

(A) Plasma concentrations of total homocysteine (Hcy), cystathionine (Cth), total cysteine (Cys), methionine (Met), and SAM/SAH ratio of the PEG-CBS-treated mice from 2 days of age up to ∼4 months of age (three times a week, s.c., 7.5 mg/kg; n = 3, hatched bars) compared to age-matched untreated mice (n = 3, white bars) and negative controls (n = 3, black bars). Columns that are significantly different from each other (p < 0.05) are indicated by having a different letter above the column, with no letter indicating non-significance. (B) A portion of the lens and adjacent eye wall imaged from the posterior aspect by confocal microscopy. In negative controls (left), zonular fibers (red) extend from the wall of the eye (white arrow) to attachment points near the lens equator (yellow arrow). Fibers then run for a few hundred micrometers across the lens surface toward the posterior pole. Untreated I278T mice (center) serve as positive controls. In these animals, zonular immunofluorescence is generally reduced, fiber density decreased, and fibers are absent from the posterior lens surface (orange arrow). In some regions, groups of fibers have broken (red arrow). In treated mice (right), staining intensity is largely restored, fiber integrity is preserved, and fibers are present on the posterior lens surface.