Skip to main content
. 2017 Dec 19;26(3):845–859. doi: 10.1016/j.ymthe.2017.12.015

Figure 1.

Figure 1

Differences in Senescence between hE-MSCs, P15, and hBM-MSCs, P8, and Comparison of Secreted Factors Using a Human Growth Factor Array

(A) Comparison of PDT between hBM-MSCs (P8) and hE-MSCs (P15). hE-MSCs had a shorter PDT than hBM-MSCs. Means with error bars for n = 3 independent experiments. The bars show SD and present plus value only. (B) FACS analysis demonstrated that expression of the S phase marker PCNA was higher in hE-MSCs than in hBM-MSCs. (C) Analysis of RTL by real-time genomic DNA PCR demonstrated that telomeres were longer in hE-MSCs than in hBM-MSCs. Means with error bars for n = 3 independent experiments. The bars show SD and present plus value only. (D) In a human cytokine array, the levels of only two cytokines, HGF and IGFBP1, were higher in the culture supernatant of hE-MSCs than in that of hBM-MSCs. (E) RTL in hBM-MSCs treated with various cytokines. Means with error bars for n = 3 independent experiments. The bars show SD and present plus value only. (F) PDT was reduced upon treatment with human rHGF. Means with error bars for n = 3 independent experiments. The bars show SD and present plus value only. (G) Interphase FISH analysis. PNA was used to detect telomeres and was visualized with Alexa Fluor 488. Twenty cells were quantified using the TeloFISH program, as described in the Materials and Methods. Means with error bars for counted 20 cells each group. The bars show SD and present plus and minus values.