Figure 2.

Ectopic Expression of SNAI1 Induces CSC Phenotype

(A) Western blot analysis for expression of critical EMT mediators and CSC transcription factors in parental colon cancer cell lines. (B) Western blot analysis and relative density quantitation of EMT biomarker and stem-cell-related transcription factors in ectopic-expressing SNAI1 cell lines compared with empty vector control cells. (C and D) Limited dilution spheroid assay comparing DLD1-SNAI1 (C) and HCT116-SNAI1 (D) cells with empty vector (Vec) control cells (data are shown as mean ± SD; n = 12; * indicated p < 0.05).