Figure 4.

SNAI1 Represses miR-145 Promoter Activity and Expression in Colorectal Cancer Cells

(A) Relative miR-145 expression level assessed by real-time PCR in parental colorectal cancer cell lines: HCT116 cells versus other four colorectal cancer cell lines (data are shown as mean ± SD; n = 3; * indicated p < 0.05). (B and C) Relative miR-145 expression level of (B) DLD1-SNAI1 cells and (C) HCT116-SNAI1 cells compared to vector control cells (data are shown as mean ± SD; n = 3; * indicated p < 0.05). (D and E) Luciferase reporter activity of (D) DLD1-SNAI1 and (E) HCT116-SNAI1 cells compared to vector control cells (data are shown as mean ± SD; n = 3; *p < 0.05).