Figure 6.

Release of TLP40 from thylakoid membranes as a result of a heat treatment. Isolated thylakoid membranes were incubated 5 min at 22°C or 42°C and then frozen in liquid nitrogen. Subsequently the membranes were disrupted with DM, and the membrane fraction and lumenal fraction, containing the released proteins, were separated by centrifugation. The proportions of membrane-bound and released TLP40 were determined with a specific antibody. As a control the content of the Rieske iron-sulfur protein was determined in the same fractions. The Rieske protein is located in thylakoid lumen and bound to the membrane via a single transmembrane anchoring span (Karnauchov et al., 1997).