Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 30;115(16):E3722–E3730. doi: 10.1073/pnas.1710480115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

PMC Copyright notice

Fig. 2. — Cytoskeletal changes in persistently activated cells. (A–D) AX2 and pten⁻ cells expressing the indicated constructs and F-actin probe RFP-LimE_Δcoil. RasC_Q62L expression changes the dynamics of F-actin waves, producing a persistent band of peripheral staining in pten⁻ cells. (Scale bars: A–C, 5 μm; D, 4 μm.) (E–H) Respective kymographs of cells in A–D. (I) High-resolution images of actin probe LimE in parental pten⁻ (Left) and RasC_Q62L/pten⁻ (Right) cells. Top shows a view of the ventral surface; Bottom shows a side profile. (Scale bars: 5 μm.) (J) Intermediate-wave F-actin wave pattern (GFP-LimE_Δcoil) phenotypes induced by RasC_Q62L expression in pten⁻ cells. (Scale bars: 5 μm.) Dox, doxycycline.