Fig. 3.
Increased lifetime of F-actin puncta in persistently activated cells. (A) Confocal section near the ventral surface of a RasCQ62L/pten− cell expressing F-actin probe-LimEΔcoil. (B) t-Stack of the cell in A showing lifetime of F-actin puncta. (C) The changes in intensity over the lifetime of 10 different F-actin puncta from cells seen in Top are traced in the graphs shown Below. Blue traces on graphs correspond to boxed puncta in Top. (D–F) Frequency distribution of puncta lifetimes. Wild-type and activated cells were treated with the indicated inhibitors for an hour before recording puncta lifetimes. Average lifetime of puncta in n = 100 different cells.