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Workflow for the enrichment and detection of KRAS proteoforms. (A) Schematic of the approach. (B) Silver-stained gel and corresponding Western blot illustrating enrichment of RAS proteins. E, eluate; FT, flow through; IN, input; MW, molecular weight markers; Std, KRAS4b standard. (C) Isotopically resolved mass spectrum of intact KRAS4b.
