Fig. 1.

Fn14 deficiency preserves renal function and reduces cell death in folic acid-induced AKI. AKI was induced by a folic acid overdose in WT and Fn14-KO mice. Mice were killed at 24 and 72 h. (A) Renal function assessed by plasma creatinine. (B) Cell death was assessed by TUNEL staining. (C) Histological injury score. Representative images of PAS staining and quantification according to a tubular damage score. Original magnification, 200×. (Scale bars: 50 µm.) (D) Immunohistochemistry of PCNA and quantification. Original magnification, 200×. Data are expressed as mean ± SEM of n = 7 mice per group. **P < 0.01; ***P < 0.001. n.s., nonsignificant.