Fig. 6.

Fn14 regulates the expression of necroptosis proteins during AKI at 72 h. (A–C) RIPK3 mRNA levels and RIPK1 and MLKL protein levels at 72 h of AKI. (D) MLKL immunofluorescence in AKI at 72 h. Representative images are shown. Magnification, 400× (scale bars: 10 μm); detail, 800× (scale bars: 5 μm). (E) Cleaved PARP protein levels in AKI at 72 h. Quantification and representative Western blots are shown. (F) Cleaved caspase-3 staining and quantification. Original magnification, 200×. (Scale bars: 50 µm.) (A–C, E, and F) Data are expressed as mean ± SEM of seven mice per group. *P < 0.05 vs. WT mice; **P < 0.01 vs. WT mice.