Fig. 4.

Inhibition of MEK-ERK1/2 signaling rescued abnormalities in Rgs5^−/− mice. (A) U0126 blocked MEK1/2 and ERK1/2 phosphorylation mediated by AB in Rgs5^−/− mice. (B) Effects of U0126 on histological changes at 2 wk after surgery. (C) The effects of U0126 on fibrosis. Left, PSR staining; Right, statistical results of fibrotic areas. (D) The effects of U0126 on hypertrophic and fibrotic markers expression induced by AB in Rgs5^−/− mice. mRNA was determined by real-time PCR analysis. Data represent typical results of three to five different experiments as mean ± SEM (n = 4–12 mice/per group). *P < 0.01 for PBS/sham values; ^#P < 0.01 for PBS/AB after AB.