Table I.
Specificity of the lanthanide effect on the ABA-inducible Em and HVA1 promoters in transiently transformed protoplasts
| Treatments
|
Promoter Fold-Induction (±se)
|
|||
|---|---|---|---|---|
| ABA | Salt | 35S | Em | HVA1 |
| μm | mm | |||
| 0 | 0 | 1.0 | 1.0 | 1.0 |
| 100 | 0 | 0.8 (0.1) | 16.6 (2.0) | 13.7 (1.0) |
| 0 | 1 LaCl3 | 1.1 (0.0) | 2.2 (0.6) | 3.2a (0.5) |
| 0 | 2 TbCl3 | 1.1 (0.1) | 4.6a (0.2) | n/ac |
| 0 | 5 LaCl3 | 1.4 (0.3) | 2.7 (0.8) | 3.8a (0.4) |
| 100 | 1 LaCl3 | 1.1 (0.1) | 32.6b (2.9) | 29.6b (5.6) |
| 100 | 2 TbCl3 | 1.2 (0.1) | 31.9b (1.5) | n/ac |
| 100 | 5 LaCl3 | 1.1 (0.4) | 38.8b (9.9) | 24.1 (5.5) |
Induction was calculated as the average ratio of relative reporter gene activities (promoter-GUS/Ubi-LUC) between treatment and control (set to unity) from three- or four-paired replicates in two independent experiments.
a
Significantly higher than control, P < 0.03 (one-sided paired t test).
b
Significantly higher than ABA treatment, P < 0.06 (one-sided paired t test).
c
n/a, Not analyzed.