Fig. 4.

BRD7 does not increase phosphorylation of GSK3β in the absence of mTOR. a TSC2 KO cells were infected with either Ad-GFP or Ad-BRD7 and treated with 10 nM torin1 for 1 h. Western blots were performed for the indicated antibodies. Short exposure (SE) and long exposure (LE) shown for pGSK3β blot. b Immunoblots of TSC2 KO cells infected with Ad-BRD7 and Ad-GFP as a control and then treated with 200 μM rapamycin for 30 min. Error bars are represented as mean ± SEM. P values were determined by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001). Each experiment was independently repeated at least three times