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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Hear Res. 2017 Dec 20;362:14–24. doi: 10.1016/j.heares.2017.12.009

Figure 1. Macrophages in the spiral ligament.

Figure 1

The images show macrophages in a whole-mount preparation of the spiral ligament collected from a young C57BL/6J mouse. The tissue was doubly stained with antibodies against CD45, a pan-leukocyte marker, and F4/80, a macrophage marker, using a method that has been previously described (Yang et al., 2015). A. Typical macrophages in the spiral ligament exhibit irregular shapes with large branches and short processes. B and C. Enlarged view of the macrophages in the area marked by the rectangle in A. B shows F4/80 immunoreactivity and C is the overlap of F4/80 and CD45 immunostaining of the same region shown in B.