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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Mar 27;518:335–348. doi: 10.1016/j.virol.2018.03.016

Figure 4. Higher amounts of dsRNA were detected during infection with ECTVΔE3L compared to w.t. ECTV.

Figure 4

(A) BS-C-1 cells were infected (MOI=10) with the indicated viruses for 24 hrs prior to staining for dsRNA (red) and cell nuclei/virus factories (DAPI; blue). Images (400x total magnification) were merged using ImageJ software and are representative of two independent trials. AraC was added at the time of infection where indicated. (B) BS-C-1 cells were infected as above prior to staining for intracellular dsRNA using flow cytometry. The depicted data are representative of two independent trials. (C) BS-C-1 cells were infected as above for various lengths of time prior to measuring dsRNA positivity using flow cytometry. The bars depict the average and the error bars represent the standard deviations of three independent trials for each time point.