Table 1.
Table depicting yield of lectin during different purification steps
| Purification steps | Total protein (mg/100 g seeds) | Hemagglutination unit (HAU) | Specific activity (HAU/mg protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 1600 | 8192 | 512.10 | 1 | 100 |
| 20–80% ammonium sulfate fraction | 750 | 4096 | 546.13 | 1.06 | 50 |
| DEAE-cellulose fraction | 455 | 4096 | 900.21 | 1.75 | 25 |
| SP-sephadex fraction | 95 | 2048 | 2155.78 | 4.20 | 25 |
At every stage of purification, lectin was agglutinated by trypsin-treated rabbit erythrocytes only