Table II.
Equilibrium dialysis-binding data
| Protein | Substrate | Kd* | bmax |
|---|---|---|---|
| μm | |||
| AN9 | Isoquercitrin | 88 | 7.2 |
| AN9 | Isoquercitrin + GSH | 66 | 4 |
| AN9 | Luteolin | 185 | 11.7 |
| AN9 | Luteolin + GSH | 121 | 9.6 |
| GSTIII | Isoquercitrin + GSH | 157 | 10.6 |
| GSTIII | Luteolin + GSH | 215 | 13.9 |
| GSTI | Isoquercitrin + GSH | 268 | 8.75 |
| GSTI | Luteolin + GSH | 142 | 15.3 |
Equilibrium dialysis-binding data. All GSTs tested bound flavonoids to a certain extent, even those that are not inhibited in their CDNB conjugating activity by flavonoids. AN9 had the highest affinity for isoquercitrin of all GSTs tested. Values are given in μm for the Kd* values, and in numbers of molecules bound per mole of protein molecule for the bmax values. The constants were determined from Lineweaver-Burk plots. The AN9 data represent the means of two experiments performed in triplicate; for the other enzymes, data are shown from one experiment performed in triplicate.