Table 2.

Primer pairs used for amplification of rickettsial genes or intergenic regions in the present study.

Primer pair	Target	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Annealing temperature (°C)	Amplicon size (nt)	Reference
(1)	mppA-purC	GCAATTATCGGTCCGAATG	TTTCATTTATTTGTCTCAAAATTCA	45 to 551	160	[33]
(2)	nusG-rplK	CAGTTGCAATATTGGTAAAGCA	CAGCAGCTGGAATTATCAAGTT	45 to 551	270	[33]
(3)	murG-RC0563	GAAGAAAAGAAGGGCATAAGCTA	CAAGCTGAAAGTAAAAACATTCC	40 to 521	293	[33]
(4)	ntrY-rpsU	AGCTGCTGTTGCTAAAGTAAAAA	CAAGAAGCAGCAAGAAGACAGA	52 to 641	363	[33]
(5)	dksA-xerC	TCCCATAGGTAATTTAGGTGTTTC	TACTACCGCATATCCAATTAAAAA	45 to 551	416	[33]
(6)	spo0J-abcT1	AAAGATTTGGAAGAATTAGACTTGAT	TTTGCTTAAACCAACCATTTCA	45 to 551	259	[33]
(7)	fabZ-lpxD	TGTTAGGATCGATTTTAAGTACTCTATCT	TGGATTGGCATAGACAATCTATTA	45 to 551	195	[33]
(8)	RC1137-tlc-5	CGGGATAACGCCGAGTAATA	ATGCCGCTCTGAATTTGTTT	45 to 551	264	[33]
(9)	RC0230-RC0231	TGCACCCGCCTAAAACTAAC	ATGGTCGGCCGTAGAAAAA	45 to 551	232	[33]
(10)	groES-RC0970	CTTGCATCGGCTTTTCTTTT	AGCTTTGAGCTGATGGGCTA	45 to 551	215	[33]
(11)	rrf-pyrH	GAGCTTTCTCCATCTTTTCTTG	AAAGGGGAATATACGACAATTGAG	45 to 551	238	[33]
(12)	tRNAGly-tRNATyr	AGCTTGGAAGGCTGGAACTC	ATCCTTCTCCCTCCACCACT	45 to 551	148	[33]
(13)	pal-RC1201	TGCAAGCACACATAATGCAA	TCAAAATCGATTCCTCTTTTCC	45 to 551	216	[33]
(14)	atpA	ATCAAGCGTTGCACAGATAG	GGAAGTGCCGTAAGTGAACC	58	Unknown∗	[1]
(15)	rpmE-tRNAfMet	TTCCGGAAATGTAGTAAATCAATC	TCAGGTTATGAGCCTGACGA	54	144	[33]
(16)	RC1027-xthA2	GGTATGTAAATGAGCCTTATCAATACT	TCAGTAGTATAAGTAGCTCCTGCTGTC	54	351	[33]
(17)	gltA	GTCTACTGCTTCGTGTAGATCAAC	GGCTGACCTATAGAATATTTATAAGAC	54	408	[25]
(18)	coxA	ACAGCCGTTGATATGGCTA	CATATTCCAACCGGCAAAAG	58	Unknown∗	[1]
(19)	coxA	GGTGCTCCTGATATGGCATT	CATATTCCAGCCGGCAAAAG	58	Unknown∗	[1]
(20)	atpA	ATCAAGCGTTGCACAGATAG	CGACTTACCGAAATACCGAC	56	449	[1, 34]

^∗Data not given in the original publication; ¹tested in gradient PCR.