Table 2.
Groups | No. embryos | 120 h Blastocysts+ | No. embryos | 120 h Blastocysts | ||
---|---|---|---|---|---|---|
No. total cells | No. Oct4 positive cells | Oct4 positive cells/Total cells (%) | No. apoptosis cells | |||
HFE | 37 | 116.0 ± 3.2a | 25.2 ± 1.2 | 21.7 ± 0.8e | 14 | 1.9 ± 0.6 |
LFE | 39 | 129.4 ± 4.4b | 24.5 ± 1.2 | 18.8 ± 0.6f | 13 | 1.2 ± 0.6 |
HPA | 49 | 105.7 ± 2.6c | 23.2 ± 1.0 | 21.9 ± 0.7e | 11 | 1.4 ± 0.4 |
LPA | 60 | 112.6 ± 2.7acd | 27.6 ± 0.9 | 24.7 ± 0.6g | 12 | 1.3 ± 0.4 |
HFE and LFE fertilized embryos cultured in 20 and 5% oxygen, HPA and LPA parthenogentic embryos cultured in 20 and 5% oxygen
+Mean ± SEM
a vs. b, p < 0.01; a vs. c, p < 0.05; b vs. c, p < 0.0001; b vs. d, p < 0.001; c vs. d, p < 0.05; e vs. f, g P < 0.01; f vs. g, P < 0.01