Figure 4.

Northern blots of RNAs isolated from: different regions of leaves of P. oceanica (1, basal; 2, central; a schematic representation of leaf is reported) collected from Antignano meadows in different months and immediately frozen in liquid nitrogen (top) or isolated from the basal region of leaves of P. oceanica collected in March, immediately frozen in liquid nitrogen (C_o) or frozen after 2 h (C_2h), 3 d (C_3d), or 15 d (C_15d) in an aquarium, hybridized with digoxygenin-labeled Pomt2b probe (bottom). Ten micrograms of total RNA per sample was loaded. A Phaseolus coccineus 25S rDNA fragment was used as a control probe (rRNA).