. Author manuscript; available in PMC: 2019 Apr 1.

Published in final edited form as: Microbes Infect. 2018 Jan 31;20(4):245–253. doi: 10.1016/j.micinf.2018.01.004

Table 1.

Primer Sequences of Known Virulence Factors for which Were Screened by in silico PCR Among 31 C. difficile REA Group DH Strains

Gene	Protein	Primer sequence (5’ ➔ 3’) [27]
tcdA	Toxin A	GCATGATAAGGCAACTTCAGTGGTA AGTTCCTCCTGCTCCATCAAATG
tcdB	Toxin B	CCAAARTGGAGTGTTACAAACAGGTG GCATTTCTCCATTCTCAGCAAAGTA GCATTTCTCCGTTTTCAGCAAAGTA
tcdC	Negative regulator of toxins A and B	AAAAGGGAGATTGTATTATGTTTTC CAATAACTTGAATAACCTTACCTTCA
cdtA	Binary toxin subunit A	GGGAAGCACTATATTAAAGCAGAAGC GGGAAACATTATATTAAAGCAGAAGC CTGGGTTAGGATTATTTACTGGACCA
cdtB	Binary toxin subunit B	TTGACCCAAAGTTGATGTCTGATTG CGGATCTCTTGCTTCAGTCTTTATAG