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. 2018 Apr 16;9:345. doi: 10.3389/fphar.2018.00345

FIGURE 4.

FIGURE 4

Ser372 phosphorylation was required for AST-induced repression of SREBP-1c transcription activity in insulin-resistant HepG2 cells. Mutation of SREBP-1c at Ser372 reversed the effect of AST on the transcription activity of SREBP-1c (A), and decreased IRS-2 transcription (B); Mutation of SREBP-1c at Ser372 reversed the effect of AST on phosphorylation of SREBP-1c (C), IRS-2 (D), and expression of FAS (E). (F,G) Representative bands from immunoblotting assays. AST, astragaloside; VEH, vehicle; IR, insulin resistance; WT, wild type. Data represent the mean ± SD as determined by one-way ANOVA, &p < 0.05, ∗p < 0.05 vs. the WT-IR+VEH group; #p < 0.05 vs. the S372A-IR+VEH group.