FIGURE 7.

Behavioral responses of auditory fear conditioning in awake and anesthetized mice. (A) Training Chamber A and the training paradigm. (B) Freezing responses (mean ± SEM) of awake (dots; n = 5, gray lines: individual) and anesthetized (circles; n = 5) mice to auditory fear conditioning (four trials). CS, 30-s noise burst at 80 dB SPL; US, 1-s footshock delivered 20 s after CS. (C) Testing Chamber B and the testing paradigm. The testing included a 3-min adaptation (pre-CS) and a 3-min CS (noise burst at 80 dB SPL). (D) Freezing responses (mean ± SEM) among awake-unconditioned (white), anesthetized-conditioned (gray) and awake-conditioned (black) groups of mice observed during the pre-CS and CS presentations 24 h after the training were compared with each other. (E) Freezing responses of the awake-conditioned mice (n = 5, black: average, gray: individual) tested over the 28 days after the training. ^∗∗∗P < 0.001. NS, not significant.