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. 2018 Mar 8;7(4):1253–1263. doi: 10.1002/cam4.1310

Figure 5.

Figure 5

UCA1 promoted CREB1 expression by regulating miR‐590‐3p expression in GC cells. (A) Alignment of potential CREB1 base pairing with miR‐590‐3p was predicted by miRanda. (B–C) The relative luciferase activities were detected by transfected with miR‐NC, miR‐590‐3p mimic, si‐UCA1, and pmirGlo‐3'UTR‐CREB1‐WT or pmirGlo‐3'UTR‐CREB1‐MUT into SGC‐7901 and MKN‐45 cells. (D) The relative mRNA expression of CREB1 was detected by transfecting with si‐NC, si‐UCA1, miR‐590‐3p inhibitor, or si‐UCA1 and miR‐590‐3p inhibitor in SGC‐7901 cells. (E) The relative mRNA expression of CREB1 was analyzed by transfection with pcDNA, pcDNA‐UCA1, miR‐590‐3p mimic, or pcDNA‐UCA1 + miR‐590‐3p mimic in MKN‐45 cells. (F) The relative protein expression of CREB1 was analyzed by transfecting with transfecting with si‐NC, si‐UCA1, miR‐590‐3p inhibitor, or si‐UCA1 and miR‐590‐3p inhibitor into SGC‐7901 cells or transfecting with pcDNA, pcDNA‐UCA1, miR‐590‐3p mimic, or pcDNA‐UCA1 + miR‐590‐3p mimic into MKN‐45 cells. The data are presented as mean ± SD from three independent experiments.*P < 0.05.