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Graphic representation showing the culture conditions of natural killer (NK) cells. During the preactivation phase (16 –18 h), cells were stimulated with different combinations of IL-12, IL-15, and IL-18 in the presence and absence of ruxolitinib. During the expansion phase, NK cells were cultured with IL-2 for 4 days. At the end of the preactivation and expansion phases, the phenotype and effector functions of NK cells were tested.
