Table 2.
Quantification of fluorescence signal ratio of on-chip purified Streptococcus pneumoniae proteins obtained by ratio of fluorescence intensity of spot to fluorescence intensity of E. coli lysate.
|
S. pneumoniae |
Streptococcus mitis |
|||
|---|---|---|---|---|
| Average | SD | Average | SD | |
| Buffer | 0.9 | 0.1 | 1.0 | 0.3 |
| E. coli_lysate | 1.0 | 0.2 | 1.0 | 0.4 |
| SP0013a | 2.1 | 0.4 | 1.9 | 0.5 |
| SP0091 | 1.0 | 0.1 | 1.0 | 0.3 |
| SP0092 | 1.4 | 0.2 | 1.1 | 0.3 |
| SP0102 | 1.2 | 0.2 | 1.1 | 0.3 |
| SP0107 | 0.9 | 0.1 | 1.1 | 0.3 |
| SP0117 | 2.0 | 0.4 | 1.4 | 0.4 |
| SP0385 | 1.2 | 0.2 | 1.4 | 0.4 |
| SP0523 | 1.2 | 0.2 | 1.0 | 0.3 |
| SP0704 | 1.1 | 0.3 | 0.9 | 0.3 |
| SP0749 | 1.1 | 0.1 | 1.0 | 0.3 |
| SP0751 | 1.1 | 0.1 | 1.1 | 0.4 |
| SP0954 | 1.2 | 0.2 | 1.0 | 0.3 |
| SP1002 | 1.3 | 0.2 | 1.2 | 0.3 |
| SP1067 | 1.0 | 0.2 | 1.1 | 0.4 |
| SP1069 | 1.0 | 0.1 | 1.2 | 0.4 |
| SP1264 | 2.1 | 0.4 | 1.2 | 0.4 |
| SP1400 | 1.1 | 0.1 | 1.0 | 0.4 |
| SP1601 | 1.2 | 0.2 | 1.1 | 0.6 |
| SP1604 | 1.5 | 0.2 | 1.0 | 0.4 |
| SP1628 | 1.3 | 0.2 | 1.2 | 0.3 |
| SP1650a | 41.3 | 6.4 | 2.4 | 0.7 |
| SP1684 | 1.3 | 0.2 | 1.1 | 0.5 |
| SP1839 | 1.1 | 0.1 | 1.1 | 0.3 |
| SP1952 | 1.4 | 0.3 | 1.3 | 0.5 |
| SP1964 | 1.3 | 0.4 | 1.2 | 0.6 |
| SP2013 | 1.3 | 0.1 | 1.3 | 0.4 |
| SP2108 | 2.7 | 0.9 | 1.2 | 0.3 |
| SP2201a | 2.7 | 0.6 | 1.6 | 0.5 |
| SP2239 | 1.1 | 0.2 | 1.0 | 0.4 |
aSignal intensity ratio > 1.5 for both S. pneumoniae and S. mitis.
Average values were calculated from three replicates.