Table 2.
Results of serology (Ab) and polymerase chain reaction (PCR) analyses for Anaplasma phagocytophilum.
| Animal ID | T1 |
T2 |
T3 |
T4 |
||||
|---|---|---|---|---|---|---|---|---|
| PCR | Ab | PCR | Ab | PCR | Ab | PCR | Ab | |
| G1 | – | + | – | – | – | – | + | – |
| G2 | – | – | – | – | – | – | – | – |
| G3 | – | + | – | + | – | – | – | – |
| G4 | – | – | – | – | – | – | – | – |
| G5 | – | + | – | – | – | – | +§ | – |
| G6 | – | – | + | – | + | – | +§ | – |
| G7 | – | – | – | – | – | – | – | – |
| G8 | – | – | – | – | – | + | – | – |
| G9 | – | + | – | – | NA | NA | – | – |
| G10 | +§ | + | + | – | + | – | +§ | – |
| n = positive (site G) | 1 | 5 | 2 | 1 | 2 | 1 | 3 | 0 |
| L1 | – | + | – | – | – | – | – | – |
| L2 | – | + | – | – | – | – | – | – |
| L3 | – | – | +§ | – | – | + | – | + |
| L4 | – | + | – | – | – | – | – | – |
| L5 | – | + | +§ | – | – | + | (+) | + |
| L6 | – | + | – | – | +§ | – | + | + |
| L7 | NA | + | +§ | + | + | + | – | + |
| L8 | – | + | NA | NA | – | + | – | + |
| L9 | – | + | – | – | – | – | – | – |
| L10 | – | + | +§ | – | – | – | – | – |
| n = positive (site L) | 0 | 9 | 4 | 1 | 2 | 4 | 2 | 5 |
| n = positive (total G + L) | 1 | 14 | 6 | 2 | 4 | 5 | 5 | 5 |
§, subjected to 16s rRNA amplicon sequencing; +, indicates weak positive PCR reaction. T1–T4, sampling occasions (see Materials and Methods); G, lambs from the island of Gotland; L, lambs from the mainland.