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Analysis of epitope specificity of LAM-reactive mAbs by ELISA to glycoconjugates expressing synthetic glycans corresponding to motifs present in ManLAM. (A) Detailed structure of glycans used in Ara4 (YB-8-099) and Ara6 (MJ-LZ-1) glycoconjugates. (B) ELISA reactivity pattern of 10 mAbs tested at the three concentrations indicated for binding activity against a glycoconjugate panel. (C) Glycan structures of additional glycoconjugates used in this assay.
